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Ice crystal formation disrupts the peptides structure, and repeated freeze-thaw cycles have been observed to reduce biological activity by 20-50% (2)
Tollens' Schiff Todoform MP 2,4-DNP A + + 245-247 C MP SCA 216-220 C B --- + + 117-119 C 110-112 C + ---- 155-156 C 164-165 C D + 190-192 C 222-223 C Note: "2,4-DNPH = 2,4-dinitrophenyl hydrazone derivative, "SCA = Semicarbazide derivative Student Report ID #2 = Unknown A- Option #1, ID #1 = Or Option #2, Redo test/ der = ID #1 = Optional - other possible ID = ID #2 - reason = _, ID #2 = Unknown B- Option #1, ID #1 - Or Option #2, Redo test/ der = ID #1 - Optional - other possible ID = ID #2 = reason = _, ID #2 = Unknown C- Option #1, ID #1 = Or Option #2, Redo test/ der = ID #1 = Optional - other possible ID = ID #2 = reason = Unknown D ID #2 = Option #1, ID #1 = Or Option #2, Redo test/ der = ID #1 = Optional - other possible ID ID #2 = reason =
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