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Next, 50 L of a 4 mM NADP + (Neta Scientific, SPCM-N1131-OM) and 20 U/mL IDH1 (Sigma-Aldrich, I1877) solution, and 50 L of 10 mM sodium citrate were sequentially added to initiate the assay

In vitro methods are thus essential for the early testing of the biosafety of newly developed cosmetic formulations before they are forwarded into skin tolerance and application tests

together with regulation of selenoprotein expression, SELENOP-dependent Se uptake to the brain seems to play an important role in maintaining this strict hierarchy

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