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At the end of the study, the rats were anaesthetized with a ketamine (10%)/xylazine (2%) mixture (80/5 mg/kg) (Alfasan, Netherlands)

It is tempting to speculate that the stimulus-specific differences in PKC phosphorylation profiles (that impact on substrate specificity) underlie PKCs diverse roles in both the pathogenesis of ischemia-reperfusion injury and as a mediator of ischemic preconditioning ( Finally, it is worth mentioning that other tyrosine residues in the regulatory region of PKC, that figure prominently in the transduction of genotoxic stress responses, can also become phosphorylated in the setting of oxidative stress ( 64 and Tyr 155 (sites in the regulatory domain) leads to a conformational change that exposes bipartite nuclear localization signal in the catalytic domain

This non-selectivity is not accidental it reflects the original design intent of producing a maximal tanning stimulus

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