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Cells are left in this environment on average for four hours, however, this depends on the cell type

doi:10.1016/j.cca.2015.08.002 Baggio E, Gandini R, Plancher AC, Passeri M, Carmosino G

Wuerstle, B

some natural compounds such as benzoic and propionic acids can also exhibit toxicity (Shaw, 2018)
