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Severe heart or liver failure and allergies to solution components

L., Lee J., Hartman A

The real-time PCR conditions were set as follows: an initial denaturation at 95 C for 5 min, followed by 40 cycles of denaturation at 94 C for 15 s, annealing at 65.5 C for 1 min, and extension at 72 C for 30 s

Freeman et al., 2009
