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Measurement of NAD(P)H autofluorescence, intracellular Ca 2+ , and mitochondrial and cytosolic glutathione oxidation After culture, islets from N5-WT and N5-Tr mice were perifused (~1 ml/min) simultaneously at 37C in a chamber placed on the stage of an inverted microscope
The staining solution was aspirated, and 100 L of nuclear solid red staining solution was introduced to each slice, with subsequent washing in water
Furthermore, direct vein injury, thrombosis, thrombocytopenia, and prolonged bleeding were all counteracted (Vukojevic et al., 2018
Vanessa Niles, M.D
