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Oxidative stress assay MDA levels Malondialdehyde (MDA) levels were determined in duplicate, following the protocol established by Mrmora et al

investigated the effects of isolated compounds from the rhizome on thrombin-induced hyperpermeability in human microvascular endothelial cells and found that spirostanol saponins and esculin reduced endothelial hyperpermeability

doi:10.2174/157340313805076322

Key among these are the nuclear factor-kappa B (NF-B) and mitogen-activated protein kinase (MAPK) pathways
