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In early development, hepatic progenitor cells emerge from the ventral foregut endoderm, progressing through various differentiation stages to become mature hepatocytes and bile duct epithelial cells
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The reconstitution itself follows the standard slow-stream technique: add diluent against the inner glass wall of the vial, swirl gently for 30 to 60 seconds until the lyophilized cake is fully dissolved, and do not shake
