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Cells were then washed, detached, and fold increased green fluorescence over baseline (stained but untreated) was determined by flow cytometry (excitation 520 nm) counting 5000 cells per sample

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In addition, Hwang et al.found that luteolin inhibited the growth of liver cancer cells and reduced tumor volume by activating AMPK, which may be related to the inhibition of NF-B activity, indicating that AMPK is a potential target for cancer prevention ( Lactobacillus species, resulting in increased apoptotic protein levels and tumor cell death, revealing the effect of Stigmasterol remodeling of intestinal flora on immune cells, which provides a theoretical basis for the clinical application of Stigmasterol in the treatment of hepatocellular carcinoma