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In brief, primary myoblasts from WT , Alix / , and ccOzz / mice were isolated and maintained on collagen Icoated dishes (BD Biosciences) in Hams F-10 medium supplemented with 20% fetal calf serum (FCS), 1 ng/mL human basic fibroblast growth factor (Promega), penicillin/streptomycin (Pen/Strep

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