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Histology (H&E), IHC (MUC2 and Nrf2), ROS (ELISA), gene expression (qRT-PCR), and protein levels (Western blotting) were analyzed in colon tissues
Glutathione plays a key role in neutralising free radicals, supporting natural liver detoxification, and maintaining healthy cellular and mitochondrial function
The selected articles reported quantitative methods to determine DNA methylation including immunocolorimetric assays for total content of genomic DNA methylation, and microarray technologies, methylation-specific quantitative PCR, Luminometric Methylation Assay (LUMA), and bisulfite pyrosequencing for DNA methylation content of genomic sites such as gene promoters, LINE-1, Alu elements, and others
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