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Description

Conditions for amplification were: 5 minutes at 95C, 40 cycles of 95C for 10 seconds and 30 seconds with 62C annealing temperature

Dispose of the used needle in a sharps container to ensure safe disposal

COS-7 cells were seeded in 12-well culture dishes at a density of 100,000 cells per well and maintained in high-glucose Dulbeccos modified Eagles medium containing 5% fetal bovine serum, 100 units/ml penicillin, and 100 g/ml streptomycin at 37 C
