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Site-directed mutagenesis was performed using a standard PCR-based protocol with S7 Fusion Polymerase (Biozym Scientific GmbH, Hessisch Oldendor, Germany)
PMID: 2706459
This sets up a vicious loop: hyperglycemia oxidative stress low GSH worse insulin resistance more hyperglycemia
Activation of FOXO3a by AZD6244 (ARRY-142886) is essential for AZD6244-induced growth suppression in human cancer cells
