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But for singers who perform regularly or are recovering from vocal strain, dietary habits alone may not be enough to keep up with demand

Hussein Department of Public Health, Wollo University, Dessie, Ethiopia Foziya Mohammed Hussien Department of Occupational Safety and Health, China Medical University, Taichung, Taiwan Bing-Fang Hwang Department of Occupational Therapy, Asia University, Taichung, Taiwan Bing-Fang Hwang Department of Health Promotion and Education, University of Ibadan, Ibadan, Nigeria Segun Emmanuel Ibitoye & Abiola Ogunkoya Department of Community Medicine, University College Hospital, Ibadan, Ibadan, Nigeria Olayinka Stephen Ilesanmi Faculty of Medicine, University of Belgrade, Belgrade, Serbia Irena M

Alternatively, we also optimized the cell sorting procedure by separating the bead/cell complexes through free settling instead of using the magnetic stand, as described previously (Yang et al., 2022)

2.4 Elisa assays Protein levels of Kim-1 and Ngal in serum and kidney tissues were quantified using commercially available ELISA kits (Elabscience, Wuhan, Hubei, China)