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(Please use sonication to dissolve the DPPH Reagent.) Measuring method After preparing the reagent, add the reagent and sample to the 96-well microplate and react for 30 minutes

Those observations are included here only to summarize research context

Vanheel H, Vicario M, Vanuytsel T, Oudenhove LV, Martinez C, Keita V, et al

Amylin receptor signaling primarily engages Gs proteins, which stimulate adenylate cyclase and increase intracellular cyclic AMP concentrations