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Characterization of the lipid acyl hydrolase activity of the major potato ( Solanum tuberosum ) tuber protein, patatin, by cloning and abundant expression in a baculovirus vector

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Abbreviations 2-NBDG: 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-deoxyglucose acc : Acetyl-CoA carboxylase alpha aco : Acyl-CoA oxidase atgl : Adipose triglyceride lipase AKT: Protein kinase B ALT: Alanine aminotransferase AST: Aspartate aminotransferase ATP: Adenosine triphosphate AMPK: Adenosine 5-monophosphate (AMP)-activated protein kinase ANOVA: Analysis of variance BCA: Bicinchoninic acid CON: Control diet CHA: Chorismate CPT1A: Carnitine palmitoyltransferase 1A CR: Carcass ratio CS: Control diet supplemented with 10 8 CFU/g C