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The supernatants were centrifuged subsequently at 2000 g at for 20 min and 10,000 g for 30 min then filtered through 0.22 m poly-styrene vacuum filters (Merck Milliporem St

An organic chemistry student guide

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The initial link between a GHRHR mutation and impaired growth was in the little mouse, where Lin et al identified an amino acid substitution in codon 60 of the mouse GHRHR (88)
