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Cells were harvested and resuspended in the same lysis buffer as for ecGFP, and lysed by passing through a Constant cell disruptor three times at 25, 28, and 30 kpsi

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Bhem suen me dojt k tomu, e st vody sublimuje (pechz pmo z pevnho skupenstv do plynnho), co zpsob, e se materil pichyt na stny vialky a vypad, jako by byla tm przdn