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Comparison of aqueous humour and serum zinc levels in humans
For live/dead detection, the cells were washed once with PBS, and then 500 L Calcein/PI Live/Dead Viability/Cytotoxicity assay kit was added to each well and incubated for 30 min at 37 C in the dark

Some studies have looked at the use of antioxidants alongside traditional light therapies to see if they improve outcomes

For HCT116, 4 NuPAGE LDS sample buffer was added to the cell lysates of (NP0007, Thermo Fisher Scientific) supplemented with 10% DTT and heated at 95 C for 5 min
