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According to the three peaks obtained after fitting the spectra of C 1s in Figure 3C, the binding energies are 285.75, 286.36, and 288.68 eV, which are from CC, CO, and C=O bonds, respectively

Furthermore, by integrating with phosphopeptide enrichment, the nanoFAC 2D RPLC platform identified 20,000 phosphopeptides from 100 g of MCF-7 cell lysate

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