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We demonstrate in a humanised FcRn/albumin double transgenic mouse model (AlbuMus) the ability to tune half-life based on the albumin sequence fused with a BiTE-like bispecific (anti-EGFR nanobody x anti-CD3 scFv) light T-cell engager (LiTE) construct [(t 0.6 h (Fc-less LiTE), t 19 hours (Albu-LiTE-NB), t 26 hours (Albu-LiTE-WT), t 37 hours (Albu-LiTE-HB)]

doi: 10.1038/s41380-020-0688-6 361 TianM.HanY.-B.YangG.-Y.LiJ.-L.ShiC.-S.TianD