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Absorbance of reaction mixture was recorded at 734 nm by Spectrophotometer (Jenway 3300, UK) and percent inhibition was calculated by the following formula: Ferric reducing antioxidant power assay (FRAP) FRAP reagent was made by adding acetate buffer (300 mM, pH 3.6), TPTZ (10 Mm) in HCl (40 Mm), and ferric chloride (0.054 g, 20 mM)

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While the production of ATP is decreased by this, and the production of oxygen radicals that cause lipid peroxidation is also simultaneously decreased

Abstract Introduction: The development of organotypic slice cultures of central nervous system (CNS) tissues has bridged the gap between simple in vitro cell cultures and complex in vivo whole animal studies