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In the Haber-Weiss cycle, OH is generated through the interaction of superoxide anion (O 2 ) and H 2 O 2
Sample tube test: take micro glass cuvette, add 20 L of sample, 140 L of Reagent II, 40 L of Reagent III in turn, mix well, and then stand for 2 minutes to test the absorbance ATat 412 nm, A = AT AB

In preliminary experiments, paraquat at 50-500 M produced ROS in Swiss 3T3 fibroblasts in a dose-dependent manner at 30-60 min

To, L., Villarreal, L
