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1755, and died April 4th, A.D

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The WBM1177 strain ( wbmIs81[eft-3p::3XFLAG::GFP::SKL::unc-54 3 untranslated region (UTR), *wbmIs65] ), which expresses a peroxisome localization sequence (SKL) fused to GFP driven by the eft-3 promoter, was generated by microinjection using clustered regularly interspaced short palindromic repeats (CRISPR) into WBM1140 ( wbmIs65 [eft-3p::3XFLAG::dpy-10 crRNA::unc-54 3 UTR ]) using the SKI LODGE system 139