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Three-Dimensional Pellet Culture of Chondrocytes For the 3D pellet culture of chondrocytes, 2.5 10 5 cells were used to form one pellet

For the xenograft model, AGTR1-overexpressing T-24 cells and control virus-infected T-24 cells were prepared at a concentration of 5.0 10 6 cells in a 1:1 mixture of 50 l of Hanks Balanced Salt Solution (Gibco) and 50 l Matrigel (Corning, Corning, NY, USA)

While the zinc oxide group showed significantly greater wound contraction than the control group on days 14 and 21, its performance was not as rapid as TCC in the initial phase

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