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The commonly recommended timing, morning on an empty stomach 30-60 minutes before eating, maximizes fat oxidation effects but may increase nausea in sensitive individuals

(PubMed) Jaeger B, Bosch AM

10.1007/s00109-010-0704-4 34 BrownM

Western blotting Cells were lysed in RIPA buffer (Solarbio, R0020) containing 1% PMSF (Solarbio, P0100)
