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Other nonenzymatic antioxidant systems, such as vitamins C and E, may also display selective, context-dependent effects rather than exerting global protein reduction, suggesting that a deeper understanding of antioxidant specificity is critical for developing targeted therapies (61, 62)

Oligohydramnios is a classic cause because amniotic fluid normally provides a protective cushion that allows free fetal movement and growth

3.5 Removal of GST affinity tag after protease cleavage in solution If the protein was digested in solution, the target protein can be purified further by re-chromatography on the glutathione Sepharose column to remove any un-cleaved fusion protein and the GST moiety