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PMC 4594018

Gabriel Diaz Enrico

After washing three times with PBS, cells were fixed with 1 mL 4% paraformaldehyde for 15 min, permeabilized with 1 mL 0.1% Triton X-100 for 10 min at room temperature, and blocked with 1 mL 5% BSA for 30 min

Besides, cell penetrating peptides seem promising to increase skin permeation of GHK and its derivatives
