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Berberine Protests the Heart from Ischemic Reperfusion Injury via Interference with Oxidative and Inflammatory Pathways

Oxidative stress is believed to play a role in numerous chronic conditions, notably cardiovascular disease [103, 104]

Kaczmarek, A., Vandenabeele, P

Met27 and the multiple Tyr residues at positions 1 and 10 , that are vulnerable to oxidation under prolonged storage in reconstituted form or under strongly oxidising conditions, producing methionine sulfoxide and tyrosine oxidation variants with potentially modified GHRHR binding affinity and reduced biological activity, Prepare and store reconstituted solutions under inert atmosphere where possible, protect from light exposure by using amber or foil-wrapped tubes, and verify Met27 and Tyr integrity by mass spectrometry before critical experiments if reconstituted solutions have been stored for extended periods