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Third, the RNAi effect was temporary, and off-target effects cannot be completely ruled out, although gene-specific fragments and the ds GFP control minimize this concern

The fluorescence spectrum of the lysate of Halo-Rac1-expressing cells, subtracted by that of the non-transfected cells, and the fluorescence spectrum of the SF650T ligand subtracted by that of the sonication buffer alone is shown in Supplementary Fig

The five-year data shows peak results at year one followed by gradual decline, suggesting a ceiling effect where continued use maintains rather than improves hair density

FIGURE 1 3 Methodology To identify key research findings on the association between ferroptosis and PTSD in existing literature, a comprehensive literature search was conducted