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RBA-GADA not confirmed by ECL assay were of low affinity To confirm our previous finding that GADA affinity is higher when positive in the ECL-GADA assay as compared with RBA-GADA alone [6, 7], GADA affinity was analysed by competitive RBA in a total of 43 samples, including 17 samples from the Action LADA study (nine GADA positive in both RBA and ECL and eight only positive for RBA-GADA) and 26 samples from the DiYA study (18 GADA positive in both RBA and ECL, and eight samples only positive for RBA-GADA)