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doi: 10.1016/j.tvjl.2011.03.005
doi: 10.1016/j.scienta.2020.109353
Cells were then washed twice with PBS and fixed in 4% PFA (Fisher Scientific 50-980-494) in PBS containing 0.1% Triton X-100 (PBST, Sigma-Aldrich T9284) for 20 min at room temperature at dark
Despite the preponderance of ubiquitylation in bioregulation, to date few cellular E3 ligase inhibitory factors have been characterized
