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The brain was removed, weighed, and washed in ice cold oxygenated (95% O 2, 5% CO 2) artificial cerebrospinal fluid (CSF) containing 118.0 mM NaCl, 3.5 mM KCl, 1.3 mM MgCl2, 26.2 mM NaHCO3, 1.0 mM NaH2PO4, 2.5 mM CaCl2, 11.0 mM glucose

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Another study used DGAT2 antisense oligonucleotides in obese mice on a methionine and cholinedeficient diet (used to model NASH), which again decreased hepatic lipid content, but resulted in increased liver damage 200