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Curr Mol Biol Rep 4: 1623, 2018
Then add L Tween 80 , mix evenly
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The oven method was as follows: 35 C held for 2 min, then increased to a temperature of 170 C at a rate of 4 C/min, held there for 4 min, followed by an increased temperature of 240 C at a rate of 3.5 C/min, and then held for 7 min
