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After incubation (10 min, 39C), the plates were centrifuged (200 x g, 5 min, 20C, Universal 320, Hettich, Tuttlingen, Germany) and the medium was replaced with pre-warmed XF Seahorse medium supplemented with glucose, sodium pyruvate and glutamine (all purchased from Agilent, Santa Clara, California, USA)

6 In the United States (U.S.), the prevalence of HCV infection between the years 1999 and 2002 was 1.6%, equating to about 4.1 million persons positive for antibody to hepatitis C (anti-HCV), 80% of whom are estimated to be viremic

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