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The solved X-ray crystal structure of tetrameric MSA-DH reveals the key catalytic residues Cys 290 and Glu 256 , accessible through opposing substrate and cofactor funnels
Roe CR, et al
Analysis of mitochondrial dynamics and morphology Cells were dyed with tetramethylrhodamine, ethyl ester (TMRE) (200 nM, Biotium, USA) mitochondrial fluorescence and captured with confocal microscopy focusing on mitochondrial-rich regions beside the cell nucleus
This results in the accumulation of cytosolic Ca 2+ followed by activation of synaptic vesicle exocytosis, and release of neurotransmitters
