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HMVECs (human microvascular endothelial cells) were procured from Lonza (Walkersville, MD, USA) and cultured in endothelial basal medium-2 (EBM-2, Lonza)

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Moreover, biochemical analyses were performed on whole brain homogenates, assessing malondialdehyde, glutathione and catalase as oxidative stress markers using standard ELISA and spectrophotometric assays

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doi: 10.1590/S0100-879X2012007500056

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The mean age of the males was 40.67 13.50 years and the mean age of the females was 38.25 16.83

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