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Indirect staining for the HIS-tag was performed using anti-HIS (Abcam catalog number S2917, RRID: AB_10641949) in combination with anti-mouse-HRP (R and D Systems catalog number MAB050H, RRID: AB_357354)

Gansu Province Education Science and Technology Innovation Program (ZX-62000002-2021-226)

Measurement of 4-HNE For immunostaining of 4-HNE-modified proteins, cochlear sections were rehydrated and incubated in Tris buffer (10 mM Tris, 1 mM EDTA, 0.05% Tween20, pH 9.0) at 90 C for 10 min for antigen retrieval

Animal studies on induced arthritis models document reduced inflammatory cell infiltration and improved limb function scores in BPC-157-treated subjects compared to untreated control groups
