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A completely opaque or particulate-laden solution may indicate: Incomplete dissolution (continue swirling) Use of the wrong solvent Peptide degradation Contamination If the solution doesnt clear after several minutes of gentle swirling, allow it to sit in the refrigerator for 510 minutes, then try again
Statistical analysis Data sets of metric variables were tested for normal distribution using Kolmogorov-Smirnov test with Lilliefors significance correction ( = 10%)
The interactions with the conserved Asn328 and several water molecules are also contributing to the stability of the C5 substrate
