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To inhibit the activity of GGTs, some mice were intratracheally (i.t.) administered GGsTop (0.5mg/kg, WakoPure, Osaka, Japan) or vehicle control on days 21, 22, and 23, according to previously published method (20)

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Structurally, semaglutide is a modified peptide analog of human GLP-1, designed to resist enzymatic degradation and prolong its biological half-life, allowing for once-weekly subcutaneous injection