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Inject both subcutaneously in the abdomen, rotating sites
Subsequently, we applied a focused refinement approach on the particles used in the reconstruction of holo-complex to improve the resolution for c-MET I and HGF I, resulting in a new cryo-EM map with resolution improved to 4.5 , which allowed us to build reliable models for the K3 and K4 domains of HGF (Supplementary Figs
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Gender may affect serum vitamin B12 concentrations, although results have been inconsistent
