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Convert vial amount to micrograms (if needed): mg 1,000 = mcg Concentration: total amount (mcg) total liquid (mL) = mcg/mL Draw volume: target amount (mcg) concentration (mcg/mL) = mL U100 units: mL 100 = syringe units Worked example (math only) If a vial is labelled 50 mg (50,000 mcg) and you add 10 mL, the concentration is 5,000 mcg/mL

Standard reconstitution (recommended): - 10 mg vial + 2 mL bacteriostatic water = 5 mg/mL concentration - At this concentration: 0.1 mL (10 units on an insulin syringe) = 0.5 mg MT-II - 0.05 mL (5 units) = 0.25 mg MT-II Alternative (simpler math): - 10 mg vial + 1 mL bacteriostatic water = 10 mg/mL concentration - At this concentration: 0.05 mL (5 units) = 0.5 mg MT-II - More concentrated, but each unit mark on the syringe represents a larger dose, making small dose adjustments harder Step-by-step mixing: 1

Evidence has shown that NNMT expression in white adipose tissue (WAT) is high in obesity-prone mice and low in obesity-resistant mice [2325] and significantly correlates with the percent fat mass in diet-induced obese mice [26]

The peptide blocks formation of reactive oxygen species, detoxifies toxic lipid peroxidation products, and prevents release of tissue-damaging free iron following injuryall critical for limiting oxidative damage during ischemic events