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Cleavage of the purification tag was performed using thrombin (Avantor) overnight at 4C while dialyzing into SEC buffer (50 mM NaCl, 20 mM TrisHCl 7.5, 25 mM choline chloride, 0.4 mM TCEP, 0.02% DDM, 0.002% CHS)

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TNF: tumor necrosis factor

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2) Group II (ATZ group

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