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J Biol Chem 277(37):3401034016 Halter F, Tarnawski A, Schmassmann APeskar B, (2001) Cyclooxygenase 2implications on maintenance of gastric mucosal integrity and ulcer healing: controversial issues and perspectives

We found that intracellular 1-MNA and NNMT protein expression levels were substantially higher in the fully differentiated adipocytes compared to the undifferentiated pre-adipocytes, which is consistent with the differentiation-dependent enhancement in NNMT mRNA levels and enzymatic activity reported previously in the 3T3-L1 cell lines.[37] The higher levels of 1-MNA and NNMT in adipocytes compared to pre-adipocytes may partially account for our observations that increasing concentrations (10 60 M) of NNMT inhibitor did not completely eliminate 1-MNA in adipocytes, but did fully suppress 1-MNA accumulation in undifferentiated pre-adipocytes

At high doses, oxidative stress and formation of toxic metabolites mediated by CYP2E1 are known as the principal pathway of toxicity induced by alcohol (ALC) and acetaminophen (APAP) 1,2,3,4