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Glass SB, Gonzalez-Fajardo L, Beringhs AOR, Lu X

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Losing the glutamate at position 3 sharply reduces its binding to IGF-binding proteins, which is why in cell culture it is reported to be roughly ten times more potent than intact IGF-1 at driving cell proliferation and hypertrophy [1]

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The solvation effect was also considered by computing single point energy with M062x/6-311++G(d,p) method and SMD solvation model on each optimized structure

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