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In the acute hippocampal slices prepared at 1, 14, and 56 days after SE induction, basal S100B secretion and S100B secretion in high-K + medium were not different at 1 and 56 days, in contrast to sham animals in which high-K + medium induced a decrease in S100B secretion

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pylori lysis buffer with protease inhibitor [20 mM Tris-HCl, pH 7.4, 1% Igepal, 150 mM NaCl, 1 mM EDTA, Protease inhibitor (MilliporeSigma)] and were frozen for 30 minutes to lyse cells

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