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AAV- Ap2 -SIRT3 or AAV- Ap2 (5 10 10 vg/mice) were administered via direct injection in the tail vein in mice at the first and the sixth week of the experiment, to generate adipose tissue-specific SIRT3 overexpression (AT-SIRT3OE) and control (AT-NC) mice, respectively

Any time of day as part of your supplement routine
