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Intestinal barrier (physical barrier, biochemical barrier, and immunological barrier) composed of epithelial cells, microbiota, immune cells, and their secretions is key for intestinal health and involved in protecting body from the penetration of harmful entities (e.g., microorganisms, luminal antigens, and luminal proinflammatory factors) to maintain a stable internal environment (7)

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Compared with conventional solvent extraction, supercritical fluid extraction (SFE) with SC-CO 2 has been reported to reduce solvent use by approximately 80%90% (140)

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