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The interaction between the E3 ligase Nedd4 and the 2 -AR is mediated via -Arr 2, which serves to link E3 ligase Nedd4 to the activated receptor indicating that ubiquitination of the 2 -AR is a prerequisite for its degradation, whereas ubiquitination of -Arr 2 is essential for receptor internalization (Shenoy et al., 2001)
Protein samples were resolved in 8% SDS-PAGE and transferred to nitrocellulose membranes as described [52]
The aforementioned data underscore the need for rigorous genotype-phenotype association studies in hypertension and other complex syndromes
