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Si han pasado ms de 5 das, se debe omitir esa dosis y administrar la siguiente segn lo programado

Immunoblotting To check PGL-1::SNAP protein expression, worms were boiled in 5x sample buffer (250 mM Tris pH 6.8, 25 mM EDTA pH 8.0, 25% glycerol, 5% SDS, 500 mM beta-mercaptoethanol), run on an SDS-PAGE gel, and transferred to PVDF

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doi: 10.1684/epd.2013.0575
